The 'pythonguifile' input appears to be a misnomer or a non-standard way of referring to Python GUI files. Standard Python GUI files typically use the '.py' extension, just like any other Python script. These files contain Python code that utilizes GUI libraries such as Tkinter, PyQt, Kivy, or wxPython to create graphical user interfaces. The code defines windows, buttons, text boxes, and other interactive elements, along with the logic that governs their behavior. When executed, these scripts launch applications with visual interfaces, allowing users to interact with the program through mouse clicks, keyboard input, and other GUI-based actions. The specific GUI library used dictates the structure and syntax of the code within the '.py' file, but the underlying principle remains the same: to create interactive applications with graphical frontends using Python.